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Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to <t>nitrocellulose.</t> The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.
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Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to <t>nitrocellulose.</t> The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.
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Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to <t>nitrocellulose.</t> The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.
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Boster Bio nitrocellulose membrane
Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to <t>nitrocellulose.</t> The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.
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Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to <t>nitrocellulose.</t> The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.
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Image Search Results


Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to nitrocellulose. The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.

Journal: Respiratory research

Article Title: Hypoxia-induced mitogenic factor (HIMF/FIZZ1/RELMα) in chronic hypoxia- and antigen-mediated pulmonary vascular remodeling.

doi: 10.1186/1465-9921-14-1

Figure Lengend Snippet: Figure 5 Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline- challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to nitrocellulose. The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.

Article Snippet: The lung and BALF samples were resolved by a 4–20% SDS-PAGE (Bio-Rad, Hercules, CA) gel and transferred onto nitrocellulose (Bio-Rad) membranes.

Techniques: Expressing, Saline, SDS Page, Bioprocessing, Western Blot, Stripping Membranes, Staining

Journal: eLife

Article Title: Transcriptional drifts associated with environmental changes in endothelial cells

doi: 10.7554/eLife.81370

Figure Lengend Snippet:

Article Snippet: Commercial assay, kit , Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit , Bio-Rad , Cat# 1704271 , .

Techniques: Recombinant, Red Blood Cell Lysis, Western Blot, Stripping, Protease Inhibitor, Modification, Purification, Sample Prep, SYBR Green Assay, Staining, Bradford Assay, Software